Gingipain aminopeptidase activities in Porphyromonas gingivalis
نویسندگان
چکیده
منابع مشابه
Generation of lys-gingipain protease activity in Porphyromonas gingivalis W50 is independent of Arg-gingipain protease activities.
Porphyromonas gingivalis, a black-pigmenting anaerobe implicated in the aetiology of periodontal disease, contains two loci, rgpA and rgpB, encoding the extracellular Arg-X specific proteases (RGPs, Arg-gingipains), and kgp, which encodes a Lys-X specific protease (KGP, Lys-gingipain). The rgpA and kgp genes encode polyproteins comprising pro-peptide and catalytic domain with large N- and C-ter...
متن کاملRole of Arg-gingipain A in virulence of Porphyromonas gingivalis.
In order to access the role of the Porphyromonas gingivalis Arg-gingipain proteases in the virulence of this organism, a mutant defective in the rgpA gene was constructed in strain 381. This mutant, MT10, displayed only 40% of the Arg-specific cysteine protease activity of the wild-type strain. In addition, MT10, as well as the recently characterized protease mutant G-102, which is defective in...
متن کاملHtrA in Porphyromonas gingivalis can regulate growth and gingipain activity under stressful environmental conditions.
In several micro-organisms, HtrA, a serine periplasmic protease, is considered an important virulence factor that plays a regulatory role in oxidative and temperature stress. The authors have previously shown that the vimA gene product is an important virulence regulator in Porphyromonas gingivalis. Further, purified recombinant VimA physically interacted with the major gingipains and the HtrA ...
متن کاملAltered gingipain maturation in vimA- and vimE-defective isogenic mutants of Porphyromonas gingivalis.
We have previously shown that gingipain activity in Porphyromonas gingivalis is modulated by the unique vimA and vimE genes. To determine if these genes had a similar phenotypic effect on protease maturation and activation, isogenic mutants defective in those genes were further characterized. Western blot analyses with antigingipain antibodies showed RgpA-, RgpB-, and Kgp-immunoreactive bands i...
متن کاملAntibody Responses to Arg-gingipain of Porphyromonas gingivalis in Periodontitis Patients
Arginine-specific cysteine proteinase (Arg-gingipain: Rgp) produced by Porphyromonas gingivalis is a major virulence factor of this periodontal pathogen, which is thought to play an important role in periodontitis by interrupting host defense mechanisms and by participating in the penetration and destruction of host connective tissues. We studied the IgG response against Rgp A components in pat...
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ژورنال
عنوان ژورنال: Biological Chemistry
سال: 2012
ISSN: 1437-4315,1431-6730
DOI: 10.1515/hsz-2012-0222